Controls for Immunocytochemistry
نویسندگان
چکیده
منابع مشابه
Specificity controls for immunocytochemistry: the antigen preadsorption test can lead to inaccurate assessment of antibody specificity.
The biomedical research community relies directly or indirectly on immunocytochemical data. Unfortunately, validation of labeling specificity is difficult. A common specificity test is the preadsorption test. This test was intended for testing crude antisera but is now frequently used to validate monoclonal and affinity purified polyclonal antibodies. Here, the authors assess the power of this ...
متن کاملTissue preparation for immunocytochemistry.
AIMS To investigate the effect of tissue preparation on immunostaining and to establish whether there is a standard tissue preparation schedule that allows optimal demonstration of all antigens. METHODS Blocks of tonsil were subjected to variations to a standard fixation, processing, and section preparation schedule. The sections were stained with five antibodies-L26 (CD20), UCHL1 (CD45RO), C...
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Immunocytochemistry, the identification of cell- or tissue-bound antigens in situ, by means of a specific antibody-antigen reaction, tagged microscopically by a visible label, has a remarkably wide range of applications. The basic techniques are straightforward and can be adapted to explore the localisation of virtually any molecule of interest to the researcher in samples of normal and/or mali...
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VLSI circuits design allows today to consider new modes of implementation for electrical controls. However, design techniques require an adaptation effort that few designers, too accustomed to the software approach, provide. The authors of this article propose to develop a methodology to guide the electrical designers towards optimal performances of control algorithms implementation. Thus, they...
متن کاملImmunocytochemistry: human neural stem cells.
Immunocytochemistry is a very powerful and fairly straightforward method for determining the presence, subcellular localization, and relative abundance of an antigen of interest, most commonly a protein, in cultured cells. This protocol presents an easy-to-follow series of steps that will enable researchers to conserve primary and secondary antibodies while getting high quality, reproducible qu...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2011
ISSN: 0022-1554,1551-5044
DOI: 10.1369/jhc.2010.956920